Rabbit IgG anti-Dog IgM (µ)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Dog IgM (µ)-HRPO, MinX none
• Biozol Catalog Number: ROC-204-4307
• Supplier Catalog Number: 204-4307
• Alternative Catalog Number: ROC-204-4307
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Canine
• Immunogen: Dog IgM mu heavy chain
• Conjugation: HRPO
• Alternative Names: rabbit anti-Dog IgM mu chain Antibody Peroxidase Conjugated, rabbit anti-Dog IgM mu chain HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Dog
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Dog IgM mu chain Peroxidase Conjugated has been tested dot blot and ELISA. This product is assayed against 1.0 ug of Dog IgM in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrat

Images

Dot Blot results of Rabbit Anti-Dog IgM Antibody Peroxidase Conjugated. Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Dog IgM-HRP at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Chemi filter.