This purified antibody has been tested in western blotting. By western blot a band approximately 30.3 kDa in size corresponding to human IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be op
Western blot using Rocklands anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc(TM) MP 4000 imaging system (Bio-Rad).
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