IL-17A Antibody Biotin Conjugated, Rabbit, Polyclonal

Catalog Number: ROC-209-406-B32
Article Name: IL-17A Antibody Biotin Conjugated, Rabbit, Polyclonal
Biozol Catalog Number: ROC-209-406-B32
Supplier Catalog Number: 209-406-B32
Alternative Catalog Number: ROC-209-406-B32
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Human
Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
Conjugation: Biotin
Alternative Names: rabbit anti-Interleukin-17A biotin conjugated antibody, rabbit anti-IL-17A biotin conjugated antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17
Clonality: Polyclonal
Concentration: 1.0 mg/ml by UV absorbance at 280 nm
NCBI: 3605
UniProt: Q16552
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Application Notes: This purified antibody has been tested in western blotting. By western blot a band approximately 30.3 kDa in size corresponding to human IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be op
Western blot using Rocklands anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc(TM) MP 4000 imaging system (Bio-Rad).