Goat IgG anti-Rabbit IgG (H+L)-unconj., MinX none

Artikelnummer: ROC-211-1102
Artikelname: Goat IgG anti-Rabbit IgG (H+L)-unconj., MinX none
Artikelnummer: ROC-211-1102
Hersteller Artikelnummer: 211-1102
Alternativnummer: ROC-211-1102
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Rabbit
Immunogen: Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat.
Konjugation: Unconjugated
Alternative Synonym: goat anti-Rabbit IgG Antibody, Gt-a-Rabbit IgG, Rabbit Antibody in Goat, Rabbit Secondary Antibody
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 10.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
Anwendungsbeschreibung: Anti-Rabbit IgG whole molecule has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimi
ELISA Results of Goat Anti-Rabbit IgG Antibody. Each well was coated in duplicate with 1.0 µg of Rabbit IgG (p/n 011-0102). The working dilution is 10,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).