Goat IgG anti-Rabbit IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Rabbit IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-211-1102
• Supplier Catalog Number: 211-1102
• Alternative Catalog Number: ROC-211-1102
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Rabbit
• Immunogen: Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in goat.
• Conjugation: Unconjugated
• Alternative Names: goat anti-Rabbit IgG Antibody, Gt-a-Rabbit IgG, Rabbit Antibody in Goat, Rabbit Secondary Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Rabbit
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:5,000 - 1:25,000
• Application Notes: Anti-Rabbit IgG whole molecule has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimi

Images

ELISA Results of Goat Anti-Rabbit IgG Antibody. Each well was coated in duplicate with 1.0 µg of Rabbit IgG (p/n 011-0102). The working dilution is 10,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).