Anti-Sheep IgG antibody has been tested by ELISA and western blot and is suitable for multiple immunoassays including immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-a
Western Blot of Donkey anti-Sheep IgG Antibody. Lane 1: Sheep IgG (p/n 013-0102). Load: 50 ng per lane. Primary antibody: Sheep antibody at 1,000 overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody (p/n 616-1302) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG.
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