Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX none

Artikelnummer: ROC-213-7102
Artikelname: Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX none
Artikelnummer: ROC-213-7102
Hersteller Artikelnummer: 213-7102
Alternativnummer: ROC-213-7102
Hersteller: Rockland Immunochemicals
Wirt: Donkey
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Sheep
Immunogen: Sheep IgG whole molecule
Konjugation: Unconjugated
Alternative Synonym: donkey anti-Sheep IgG Antibody, donkey anti-Sheep IgG Antibody
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 10.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Sheep
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: Anti-Sheep IgG antibody has been tested by ELISA and western blot and is suitable for multiple immunoassays including immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-a
Western Blot of Donkey anti-Sheep IgG Antibody. Lane 1: Sheep IgG (p/n 013-0102). Load: 50 ng per lane. Primary antibody: Sheep antibody at 1,000 overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody (p/n 616-1302) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG.