Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Donkey IgG anti-Sheep IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-213-7102
• Supplier Catalog Number: 213-7102
• Alternative Catalog Number: ROC-213-7102
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Sheep
• Immunogen: Sheep IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: donkey anti-Sheep IgG Antibody, donkey anti-Sheep IgG Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Sheep
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Sheep IgG antibody has been tested by ELISA and western blot and is suitable for multiple immunoassays including immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-a

Images

Western Blot of Donkey anti-Sheep IgG Antibody. Lane 1: Sheep IgG (p/n 013-0102). Load: 50 ng per lane. Primary antibody: Sheep antibody at 1,000 overnight at 4C. Secondary antibody: Peroxidase donkey secondary antibody (p/n 616-1302) at 1:40,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG.