CHK2 phospho T68 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-280
Artikelname: CHK2 phospho T68 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-280
Hersteller Artikelnummer: 600-401-280
Alternativnummer: ROC-600-401-280
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 50-75 of Human CHK2.
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-CHK2 pT68 Antibody, Serine/threonine-protein kinase Chk2, CHK-2, CHK 2, CDS1, RAD53, CHEK2, Checkpoint kinase 2, Hucds1
Klonalität: Polyclonal
Konzentration: 1.0mg/ml by UV absorbance at 280 nm
NCBI: 11200
UniProt: O96017
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:70,000, IP: 1:100, WB: 1:200 - 1:2,000
Anwendungsbeschreibung: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 by western blotting in the appr
Western blot using Rocklands Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kDa corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin. Chk2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.