CHK2 phospho T68 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: CHK2 phospho T68 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-280
• Supplier Catalog Number: 600-401-280
• Alternative Catalog Number: ROC-600-401-280
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 50-75 of Human CHK2.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-CHK2 pT68 Antibody, Serine/threonine-protein kinase Chk2, CHK-2, CHK 2, CDS1, RAD53, CHEK2, Checkpoint kinase 2, Hucds1

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0mg/ml by UV absorbance at 280 nm
• NCBI: 11200
• UniProt: O96017
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:70,000, IP: 1:100, WB: 1:200 - 1:2,000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 by western blotting in the appr

Images

Western blot using Rocklands Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kDa corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin. Chk2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.