PAK 1/2/3 phospho T423 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-413
Artikelname: PAK 1/2/3 phospho T423 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-413
Hersteller Artikelnummer: 600-401-413
Alternativnummer: ROC-600-401-413
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: DOT, ELISA
Spezies Reaktivität: Human
Immunogen: Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-PAK pT423 antibody, rabbit anti-PAK1/2/3 pT423 antibody, PAK1, PAK-1, PAK 1, Alpha PAK, p21-activated kinase 1, p65-PAK, PAK2, PAK-2, PAK 2, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, Gamma PAK, PAK3, PAK-3, PAK 3, Beta PAK, CDC42/RAC effector kinase PAK-B, p21-activated kinase 3
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 5058
UniProt: Q13153
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung: This phospho specific polyclonal antibody was tested by Dot blot and ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not
Dot Blot of Rabbit anti-PAK 1/2/3 pT423 antibody. Antigen: phosphorylated and non-phosphorylated forms of the immunizing peptide. Load: 200 ng, 66.7 ng, 22.2 ng, 7.4 ng, or 2.5 ng as indicated. Primary antibody: PAK 1/2/3 pT423 antibody at 1:1,000 overnight at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody (p/n 611-141-002) at 1:40,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 60 min at RT.