PAK 1/2/3 phospho T423 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: PAK 1/2/3 phospho T423 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-413
• Supplier Catalog Number: 600-401-413
• Alternative Catalog Number: ROC-600-401-413
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: DOT, ELISA
• Species Reactivity: Human
• Immunogen: Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-PAK pT423 antibody, rabbit anti-PAK1/2/3 pT423 antibody, PAK1, PAK-1, PAK 1, Alpha PAK, p21-activated kinase 1, p65-PAK, PAK2, PAK-2, PAK 2, PAK65, S6/H4 kinase, p21-activated kinase 2, p58, Gamma PAK, PAK3, PAK-3, PAK 3, Beta PAK, CDC42/RAC effector kinase PAK-B, p21-activated kinase 3

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 5058
• UniProt: Q13153
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:4,000 - 1:20,000, WB: 1:500 - 1:2,000
• Application Notes: This phospho specific polyclonal antibody was tested by Dot blot and ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not

Images

Dot Blot of Rabbit anti-PAK 1/2/3 pT423 antibody. Antigen: phosphorylated and non-phosphorylated forms of the immunizing peptide. Load: 200 ng, 66.7 ng, 22.2 ng, 7.4 ng, or 2.5 ng as indicated. Primary antibody: PAK 1/2/3 pT423 antibody at 1:1,000 overnight at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody (p/n 611-141-002) at 1:40,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 60 min at RT.