KLH Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: KLH Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-466
• Hersteller Artikelnummer: 600-401-466
• Alternativnummer: ROC-600-401-466
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Mollusc
• Immunogen: KLH (Keyhole Limpet Hemocyanin)
• Konjugation: Unconjugated
• Alternative Synonym: rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Anti-KLH Antibody has been tested for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Bilder

Western Blot of Rabbit anti-KLH antibody. Lane 1: Molecular Weight Marker. Lane 2: KLH. Load: 10 µg. Primary antibody: Anti-KLH at 1:1000 in 1% BSA-TBS, overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TTBS for 30 min RT. Predicted/Observed size: ~350-390 kDa for KLH.