KLH Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: KLH Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-466
• Supplier Catalog Number: 600-401-466
• Alternative Catalog Number: ROC-600-401-466
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mollusc
• Immunogen: KLH (Keyhole Limpet Hemocyanin)
• Conjugation: Unconjugated
• Alternative Names: rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-KLH Antibody has been tested for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Images

Western Blot of Rabbit anti-KLH antibody. Lane 1: Molecular Weight Marker. Lane 2: KLH. Load: 10 µg. Primary antibody: Anti-KLH at 1:1000 in 1% BSA-TBS, overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TTBS for 30 min RT. Predicted/Observed size: ~350-390 kDa for KLH.