Protein Kinase C Beta Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Protein Kinase C Beta Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-A76S
• Hersteller Artikelnummer: 600-401-A76S
• Alternativnummer: ROC-600-401-A76S
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Protein kinase C beta antibody, PKC ß, Protein kinase C beta type, PRKCB, PKCB, PRKCB1, PKC-beta, PKC-B

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 5579
• UniProt: P05771
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:75,000 - 1:80,000, WB: 1:1,000 - 1:2,000
• Anwendungsbeschreibung: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the

Bilder

Western blot using Rocklands affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 µg of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kDa PKC beta. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BSA in TTBS at 4 C, overnight. Personal Communication, Howard Fine and Svetlana Kotliarova, CCR-NCI, Bethesda, MD.