Protein Kinase C Beta Antibody, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-600-401-A76S
Supplier Catalog Number:
600-401-A76S
Alternative Catalog Number:
ROC-600-401-A76S
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, WB
Species Reactivity:
Human
Immunogen:
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-Protein kinase C beta antibody, PKC ß, Protein kinase C beta type, PRKCB, PKCB, PRKCB1, PKC-beta, PKC-B
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:75,000 - 1:80,000, WB: 1:1,000 - 1:2,000
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the
Western blot using Rocklands affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 µg of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kDa PKC beta. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BSA in TTBS at 4 C, overnight. Personal Communication, Howard Fine and Svetlana Kotliarova, CCR-NCI, Bethesda, MD.
* VAT and and shipping costs not included. Errors and price changes excepted
