HES5 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HES5 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-BR0
• Hersteller Artikelnummer: 600-401-BR0
• Alternativnummer: ROC-600-401-BR0
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-HES5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid peptide near the N-terminus of human HES5.
• Konjugation: Unconjugated
• Alternative Synonym: Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 388585
• UniProt: Q5TA89
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:20,000, IHC: 5µg/mL, IF Microscopy: 20µg/mL, WB: 1-2 µg/mL
• Anwendungsbeschreibung: Anti-HES5 Antibody has been tested for use in ELISA, immunofluorescence, immunohistochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 18 kDa in Western Blots of specifi

Bilder

Western Blot of Rabbit anti-HES5 antibody. Lane A: Raji cell lysate in the absence of blocking peptide. Lane B: Raji cell lysate in the presence of blocking peptide. Primary antibody: HES5 antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 18 kDa, 17 kDa for HES5.