HES5 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: HES5 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-BR0
• Supplier Catalog Number: 600-401-BR0
• Alternative Catalog Number: ROC-600-401-BR0
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-HES5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid peptide near the N-terminus of human HES5.
• Conjugation: Unconjugated
• Alternative Names: Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 388585
• UniProt: Q5TA89
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 5µg/mL, IF Microscopy: 20µg/mL, WB: 1-2 µg/mL
• Application Notes: Anti-HES5 Antibody has been tested for use in ELISA, immunofluorescence, immunohistochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 18 kDa in Western Blots of specifi

Images

Western Blot of Rabbit anti-HES5 antibody. Lane A: Raji cell lysate in the absence of blocking peptide. Lane B: Raji cell lysate in the presence of blocking peptide. Primary antibody: HES5 antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 18 kDa, 17 kDa for HES5.