DOPA Decarboxylase Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-C74
Artikelname: DOPA Decarboxylase Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-C74
Hersteller Artikelnummer: 600-401-C74
Alternativnummer: ROC-600-401-C74
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Rat
Immunogen: Anti-DOPA Decarboxylase Antibody was produced in rabbit by repeated immunizations with purified from inclusion bodies and SDS denatured recombinant bovine aromatic DOPA decarboxylase expressed in E. coli.
Konjugation: Unconjugated
Alternative Synonym: Aromatic-L-amino-acid decarboxylase, DDC, AADC
Klonalität: Polyclonal
NCBI: 280762
UniProt: P27718
Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Formulierung: Liquid
Target-Kategorie: Bovine
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:10,000, WB: 1:1000
Anwendungsbeschreibung: Anti-DOPA Decarboxylase Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to the DDC proteins in the appropriate c
Western Blot of Rabbit anti-DOPA Decarboxylase Bovine Antibody. Lane 1: rat adrenal medulla. Lane 2: none. Load: 10 µg per lane. Primary antibody: DOPA Decarboxylase Bovine antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~55kDa/~55kDa for DDC protein. Other band(s): none.