Anti-DOPA Decarboxylase Antibody was produced in rabbit by repeated immunizations with purified from inclusion bodies and SDS denatured recombinant bovine aromatic DOPA decarboxylase expressed in E. coli.
Anti-DOPA Decarboxylase Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to the DDC proteins in the appropriate c
Western Blot of Rabbit anti-DOPA Decarboxylase Bovine Antibody. Lane 1: rat adrenal medulla. Lane 2: none. Load: 10 µg per lane. Primary antibody: DOPA Decarboxylase Bovine antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~55kDa/~55kDa for DDC protein. Other band(s): none.
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