Anti-Tryptophan Hydroxylase pS58 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.
Anti-Tryptophan Hydroxylase pS58 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase protei
Western Blot of Rabbit anti-Tryptophan Hydroxylase Ser58 antibody. Lane 1: Recombinant tryptophan hydroxylase incubated in the absence of cAMP-dependent protein kinase (Control). Lane 2: Recombinant tryptophan hydroxylase incubated in the presence of cAMP-dependent protein kinase (PKA). Load: 10 µg per lane. Primary antibody: Tryptophan Hydroxylase Ser58 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 53 kDa for Tryptophan Hydroxylase Ser58. Other band(s): Tryptophan Hydroxylase Ser58 splice variants and isoforms.
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