Tryptophan Hydroxylase 1 phospho S58 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-C97
Article Name: Tryptophan Hydroxylase 1 phospho S58 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-C97
Supplier Catalog Number: 600-401-C97
Alternative Catalog Number: ROC-600-401-C97
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Rabbit
Immunogen: Anti-Tryptophan Hydroxylase pS58 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.
Conjugation: Unconjugated
Alternative Names: Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1
Clonality: Polyclonal
NCBI: 24848
UniProt: P09810
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Form: Liquid
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: WB: 1:1000
Application Notes: Anti-Tryptophan Hydroxylase pS58 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to tryptophan hydroxylase protei
Western Blot of Rabbit anti-Tryptophan Hydroxylase Ser58 antibody. Lane 1: Recombinant tryptophan hydroxylase incubated in the absence of cAMP-dependent protein kinase (Control). Lane 2: Recombinant tryptophan hydroxylase incubated in the presence of cAMP-dependent protein kinase (PKA). Load: 10 µg per lane. Primary antibody: Tryptophan Hydroxylase Ser58 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 53 kDa for Tryptophan Hydroxylase Ser58. Other band(s): Tryptophan Hydroxylase Ser58 splice variants and isoforms.