Anti-LIN54 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the C-terminus of human LIN54.
Anti-LIN54 Antibody has been tested for use in ELISA, immunocytochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 79 kDa in Western Blots of specifi
Western Blot of Rabbit Anti-LIN54 antibody. Lane A: HeLa cell lysate in the absence of blocking peptide. Lane B: HeLa cell lysate in the presence of blocking peptide. Primary antibody: LIN54 antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 88 kDa, 82 kDa for LIN54.
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