LIN54 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-CL1
Article Name: LIN54 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-CL1
Supplier Catalog Number: 600-401-CL1
Alternative Catalog Number: ROC-600-401-CL1
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Anti-LIN54 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the C-terminus of human LIN54.
Conjugation: Unconjugated
Alternative Names: LIN-54, JC8.6, CXCDC1, MIP120
Clonality: Polyclonal
Concentration: 1.0 mg/ml by UV absorbance at 280 nm
NCBI: 132660
UniProt: Q6MZP7
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 2.5 µg/mL, IF Microscopy: 20 µg/mL, WB: 1-2 µg/mL
Application Notes: Anti-LIN54 Antibody has been tested for use in ELISA, immunocytochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 79 kDa in Western Blots of specifi
Western Blot of Rabbit Anti-LIN54 antibody. Lane A: HeLa cell lysate in the absence of blocking peptide. Lane B: HeLa cell lysate in the presence of blocking peptide. Primary antibody: LIN54 antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 88 kDa, 82 kDa for LIN54.