Nephrin Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Nephrin Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-CZ8
• Hersteller Artikelnummer: 600-401-CZ8
• Alternativnummer: ROC-600-401-CZ8
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Anti-Nephrin antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the C-terminus of human Nephrin.
• Konjugation: Unconjugated
• Alternative Synonym: NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 4648
• UniProt: O60500
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:20,000, IHC: 5µg/mL, IF Microscopy: 20µg/mL, WB: 1-2 µg/mL
• Anwendungsbeschreibung: Anti-Nephrin Antibody has been tested for use in ELISA, Western Blotting, immunofluorescence, and immunohistochemistry. Expect a band at approximately 135 kDa in Western Blots of specific cell lysates and tissues. Western Blot tested in human and mouse s

Bilder

Western Blot of Rabbit anti-NEPHRIN antibody. Lane A: human kidney tissue lysate in the absence of blocking peptide. Lane B: human kidney tissue lysate in the presence of blocking peptide. Primary antibody: NEPHRIN antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 94 kDa, 100 kDa for NEPHRIN.