Nephrin Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Nephrin Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-CZ8
• Supplier Catalog Number: 600-401-CZ8
• Alternative Catalog Number: ROC-600-401-CZ8
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Anti-Nephrin antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the C-terminus of human Nephrin.
• Conjugation: Unconjugated
• Alternative Names: NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 4648
• UniProt: O60500
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 5µg/mL, IF Microscopy: 20µg/mL, WB: 1-2 µg/mL
• Application Notes: Anti-Nephrin Antibody has been tested for use in ELISA, Western Blotting, immunofluorescence, and immunohistochemistry. Expect a band at approximately 135 kDa in Western Blots of specific cell lysates and tissues. Western Blot tested in human and mouse s

Images

Western Blot of Rabbit anti-NEPHRIN antibody. Lane A: human kidney tissue lysate in the absence of blocking peptide. Lane B: human kidney tissue lysate in the presence of blocking peptide. Primary antibody: NEPHRIN antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 94 kDa, 100 kDa for NEPHRIN.