Anti-Elk-1 pS383 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH.
Anti-Elk-1 pS383 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to Elk-1 phosphorylated at Ser383 in the approp
Western Blot of Anti-Elk-1 pS383 (Rabbit) Antibody. Lane 1: recombinant Elk-1 non-phospho. Lane 2: recombinant Elk-1 phospho. Load: 10 µg per lane. Primary antibody: Elk-1 pS383 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~46kDa/~46kDa for Elk-1 phosphorylated at Ser383. Other band(s): none.
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