Elk-1 phospho S383 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Elk-1 phospho S383 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-D34
• Supplier Catalog Number: 600-401-D34
• Alternative Catalog Number: ROC-600-401-D34
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Mouse, Rat
• Immunogen: Anti-Elk-1 pS383 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: ETS domain-containing protein Elk-1

Product Properties

• Clonality: Polyclonal
• NCBI: 2002
• UniProt: P19419
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10000, WB: 1:1000
• Application Notes: Anti-Elk-1 pS383 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to Elk-1 phosphorylated at Ser383 in the approp

Images

Western Blot of Anti-Elk-1 pS383 (Rabbit) Antibody. Lane 1: recombinant Elk-1 non-phospho. Lane 2: recombinant Elk-1 phospho. Load: 10 µg per lane. Primary antibody: Elk-1 pS383 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~46kDa/~46kDa for Elk-1 phosphorylated at Ser383. Other band(s): none.