GABA(A) Receptor alpha 2 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-D45
Artikelname: GABA(A) Receptor alpha 2 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-D45
Hersteller Artikelnummer: 600-401-D45
Alternativnummer: ROC-600-401-D45
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Mouse, Rat
Immunogen: Anti-GABA(A) Receptor alpha 2 Antibody was produced in rabbit by repeated immunizations with a fusion protein from the cytoplasmic loop of the rat receptor alpha 2 subunit.
Konjugation: Unconjugated
Alternative Synonym: Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2
Klonalität: Polyclonal
Konzentration: Titrated value sufficient to run approximately 10 mini blots.
NCBI: 61856
UniProt: P23576
Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Formulierung: Liquid
Target-Kategorie: Rat
Antibody Type: Primary Antibody
Application Verdünnung: WB: 1:1000
Anwendungsbeschreibung: Anti-GABA(A) Receptor alpha 2 (Rabbit) Antibody is tested for use in Western Blot and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 2 subunit of the G
Western Blot of Anti-GABA(A) Receptor alpha 2 (Rabbit) Antibody. Lane 1: mouse brain lysates from wild type. Lane 2: mouse brain lysates from alpha2-knockout (alpha2-K/O). Load: 10 µg per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~51kDa/~51kDa for alpha2-subunit of the GABAA-R. Other band(s): none.