GABA(A) Receptor alpha 2 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: GABA(A) Receptor alpha 2 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-D45
• Supplier Catalog Number: 600-401-D45
• Alternative Catalog Number: ROC-600-401-D45
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Mouse, Rat
• Immunogen: Anti-GABA(A) Receptor alpha 2 Antibody was produced in rabbit by repeated immunizations with a fusion protein from the cytoplasmic loop of the rat receptor alpha 2 subunit.
• Conjugation: Unconjugated
• Alternative Names: Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 61856
• UniProt: P23576
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-GABA(A) Receptor alpha 2 (Rabbit) Antibody is tested for use in Western Blot and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 2 subunit of the G

Images

Western Blot of Anti-GABA(A) Receptor alpha 2 (Rabbit) Antibody. Lane 1: mouse brain lysates from wild type. Lane 2: mouse brain lysates from alpha2-knockout (alpha2-K/O). Load: 10 µg per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~51kDa/~51kDa for alpha2-subunit of the GABAA-R. Other band(s): none.