Anti-GABA(A) Receptor alpha 6 Antibody was produced in rabbit by repeated immunizations with recombinant fusion proteins from the cytoplasmic loop of the alpha 6 subunit.
Anti-GABA(A) Receptor alpha 6 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 57 kDa in size corresponding to the alpha 6 subunit of the GABA(
Western Blot of Rabbit anti-GABA(A) Receptor alpha 6 antibody. Lane 1: mouse forebrain lysates from Wild Type. Lane 2: mouse forebrain lysates from Wild Type alpha6-knockout (alpha6-K/O). Load: 10 µg per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~57kDa/~56kDa for alpha6-subunit of the GABAA-R. Other band(s): none.
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