GABA(A) Receptor alpha 6 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-D48
Article Name: GABA(A) Receptor alpha 6 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-D48
Supplier Catalog Number: 600-401-D48
Alternative Catalog Number: ROC-600-401-D48
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Mouse, Rat
Immunogen: Anti-GABA(A) Receptor alpha 6 Antibody was produced in rabbit by repeated immunizations with recombinant fusion proteins from the cytoplasmic loop of the alpha 6 subunit.
Conjugation: Unconjugated
Alternative Names: Gamma-aminobutyric acid receptor subunit alpha-6, GABA(A) receptor subunit alpha-6
Clonality: Polyclonal
NCBI: 29708
UniProt: P30191
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Form: Liquid
Target: Rat
Antibody Type: Primary Antibody
Application Dilute: WB: 1:1000
Application Notes: Anti-GABA(A) Receptor alpha 6 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 57 kDa in size corresponding to the alpha 6 subunit of the GABA(
Western Blot of Rabbit anti-GABA(A) Receptor alpha 6 antibody. Lane 1: mouse forebrain lysates from Wild Type. Lane 2: mouse forebrain lysates from Wild Type alpha6-knockout (alpha6-K/O). Load: 10 µg per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~57kDa/~56kDa for alpha6-subunit of the GABAA-R. Other band(s): none.