Anti-Rim3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the N-terminus of human Rim3.
Anti-Rim3 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 33 kDa in Western Blots of specific
Western Blot of Rim3 antibody. Lane A: Human brain tissue lysate in the absence of blocking peptide at 1 µg/mL. Lane B: Human brain tissue lysate in the presence of blocking peptide at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 32.7 kDa, ~39 kDa for Rim3.
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