RIM3 Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-EA1
Article Name: RIM3 Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-EA1
Supplier Catalog Number: 600-401-EA1
Alternative Catalog Number: ROC-600-401-EA1
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC, WB
Species Reactivity: Human, Mouse
Immunogen: Anti-Rim3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide from near the N-terminus of human Rim3.
Conjugation: Unconjugated
Alternative Names: Rim3 Antibody, NIM3, RIM3, KIAA0237, Regulating synaptic membrane exocytosis protein 3, RIM3 gamma, Nim3
Clonality: Polyclonal
Concentration: 1 mg/mL by UV absorbance at 280 nm
NCBI: 9783
UniProt: Q9UJD0
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:10,000, IHC: 2.5 µg/mL, IF Microscopy: 5 µg/mL, WB: 1 µg/mL
Application Notes: Anti-Rim3 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 33 kDa in Western Blots of specific
Western Blot of Rim3 antibody. Lane A: Human brain tissue lysate in the absence of blocking peptide at 1 µg/mL. Lane B: Human brain tissue lysate in the presence of blocking peptide at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 32.7 kDa, ~39 kDa for Rim3.