HDAC1 (near C-terminus) Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-J16
Artikelname: HDAC1 (near C-terminus) Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-J16
Hersteller Artikelnummer: 600-401-J16
Alternativnummer: ROC-600-401-J16
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human, Mouse, Primate, Rat
Immunogen: HDAC1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human HDAC1.
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-HDAC-1 antibody, HDAC1, HDAC 1, HD 1 antibody, histone deacetylase 1 antibody, Histone deacetylase-1, HD1 antibody, RPD3L1, reduced potassium dependency yeast homolog like 1 antibody
Klonalität: Polyclonal
Konzentration: 0.59 mg/ml by UV absorbance at 280 nm
NCBI: 3065
UniProt: Q13547
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF Microscopy: 1:100-1:500, WB: 1 µg/ml
Anwendungsbeschreibung: Anti-HDAC1 antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62 kDa corresponding to the appropriate cell lysate or extract.
Western Blot of Rabbit Anti-HDAC1 antibody. Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 5 µg per lane. Primary antibody: HDAC1 antibody at 1µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 62 kDa for HDAC1.