HDAC1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human HDAC1.
Anti-HDAC1 antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62 kDa corresponding to the appropriate cell lysate or extract.
Western Blot of Rabbit Anti-HDAC1 antibody. Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 5 µg per lane. Primary antibody: HDAC1 antibody at 1µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 62 kDa for HDAC1.
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