HDAC1 (near C-terminus) Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-J16
Article Name: HDAC1 (near C-terminus) Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-J16
Supplier Catalog Number: 600-401-J16
Alternative Catalog Number: ROC-600-401-J16
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Human, Mouse, Primate, Rat
Immunogen: HDAC1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human HDAC1.
Conjugation: Unconjugated
Alternative Names: rabbit anti-HDAC-1 antibody, HDAC1, HDAC 1, HD 1 antibody, histone deacetylase 1 antibody, Histone deacetylase-1, HD1 antibody, RPD3L1, reduced potassium dependency yeast homolog like 1 antibody
Clonality: Polyclonal
Concentration: 0.59 mg/ml by UV absorbance at 280 nm
NCBI: 3065
UniProt: Q13547
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF Microscopy: 1:100-1:500, WB: 1 µg/ml
Application Notes: Anti-HDAC1 antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62 kDa corresponding to the appropriate cell lysate or extract.
Western Blot of Rabbit Anti-HDAC1 antibody. Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 5 µg per lane. Primary antibody: HDAC1 antibody at 1µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 62 kDa for HDAC1.