Huntington Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Huntington Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-J23
• Hersteller Artikelnummer: 600-401-J23
• Alternativnummer: ROC-600-401-J23
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Huntington antibody, rabbit anti-Huntingtin antibody, HD protein, HTT, Huntington Disease Protein, Huntington disease, IT15, LOMARS

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.1 mg/ml by UV absorbance at 280 nm
• NCBI: 3064
• UniProt: P42858
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF Microscopy: 1:100-1:500, WB: 0.1 µg/ml
• Anwendungsbeschreibung: Anti-Huntingtin antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.

Bilder

Western Blot of Rabbit Anti-Huntington antibody. Lane 1: mouse brain extract absence of blocking peptide. Lane 2: mouse brain extract with blocking peptide. Load: 10 µg per lane. Primary antibody: Huntington antibody at 0.1µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 350 kDa for Huntington. Other band(s): Huntington splice variants and isoforms.