Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.
Anti-Huntingtin antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.
Western Blot of Rabbit Anti-Huntington antibody. Lane 1: mouse brain extract absence of blocking peptide. Lane 2: mouse brain extract with blocking peptide. Load: 10 µg per lane. Primary antibody: Huntington antibody at 0.1µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 350 kDa for Huntington. Other band(s): Huntington splice variants and isoforms.
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