APRIL Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-600-401-Y53
Artikelname: APRIL Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-600-401-Y53
Hersteller Artikelnummer: 600-401-Y53
Alternativnummer: ROC-600-401-Y53
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Anti-APRIL antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the internal region of human APRIL.
Konjugation: Unconjugated
Alternative Synonym: APRIL Antibody, APRIL, CD256, TALL2, ZTNF2, TALL-2, TRDL-1, UNQ383/PRO715, APRIL, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand
Klonalität: Polyclonal
Konzentration: 1 mg/mL by UV absorbance at 280 nm
NCBI: 8741
UniProt: O75888
Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:50,000 - 1:100,000, WB: 5 - 10 µg/mL
Anwendungsbeschreibung: Anti-APRIL Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 27 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of APRIL antibody. Lane 1: K562 cells with APRIL at 5 µg/mL. Lane 2: K562 cells with APRIL at 10 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27.4 kDa, 26 kDa for APRIL. Other band(s): APRIL splice variants and isoforms.