Anti-APRIL antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the internal region of human APRIL.
Conjugation:
Unconjugated
Alternative Names:
APRIL Antibody, APRIL, CD256, TALL2, ZTNF2, TALL-2, TRDL-1, UNQ383/PRO715, APRIL, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:50,000 - 1:100,000, WB: 5 - 10 µg/mL
Application Notes:
Anti-APRIL Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 27 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of APRIL antibody. Lane 1: K562 cells with APRIL at 5 µg/mL. Lane 2: K562 cells with APRIL at 10 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27.4 kDa, 26 kDa for APRIL. Other band(s): APRIL splice variants and isoforms.
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