Collagen Type VI Antibody Biotin Conjugated, Rabbit, Polyclonal

Artikelnummer: ROC-600-406-108
Artikelname: Collagen Type VI Antibody Biotin Conjugated, Rabbit, Polyclonal
Artikelnummer: ROC-600-406-108
Hersteller Artikelnummer: 600-406-108
Alternativnummer: ROC-600-406-108
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Spezies Reaktivität: Bovine, Human
Immunogen: Collagen Type VI from human and bovine placenta
Konjugation: Biotin
Alternative Synonym: rabbit anti-Collagen Type VI antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 1291
UniProt: P12109
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Mammalian
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:5,000 - 1:10,000
Anwendungsbeschreibung: Anti-Collagen Type VI (RABBIT) Antibody Biotin Conjugated is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
Immunohistochemistry of Rabbit anti-collagen VI antibody (600-401-108). Tissue: human placenta (left), testis (right). Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes. Primary antibody: collagen VI antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: collagen VI is extracellular. Staining: collagen VI as precipitated red signal red staining of stromal and extracellular spaces in the placenta and extracellular spaces between seminiferous tubules in the testis, with hematoxylin purple nuclear counterstain.