Collagen Type VI Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Collagen Type VI Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-406-108
• Supplier Catalog Number: 600-406-108
• Alternative Catalog Number: ROC-600-406-108
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: Bovine, Human
• Immunogen: Collagen Type VI from human and bovine placenta
• Conjugation: Biotin
• Alternative Names: rabbit anti-Collagen Type VI antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type VI antibody, Collagen alpha-1 (VI) chain, Collagen VI antibody, Human mRNA for collagen VI alpha 1 C terminal globular domain antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 1291
• UniProt: P12109
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mammalian
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:5,000 - 1:10,000
• Application Notes: Anti-Collagen Type VI (RABBIT) Antibody Biotin Conjugated is suitable for western blot, immunoprecipitation, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Images

Immunohistochemistry of Rabbit anti-collagen VI antibody (600-401-108). Tissue: human placenta (left), testis (right). Fixation: formalin fixed paraffin embedded. Antigen retrieval: steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes. Primary antibody: collagen VI antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: collagen VI is extracellular. Staining: collagen VI as precipitated red signal red staining of stromal and extracellular spaces in the placenta and extracellular spaces between seminiferous tubules in the testis, with hematoxylin purple nuclear counterstain.