0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Bovine
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500
Anwendungsbeschreibung:
Anti-Bovine IgG Peroxidase Conjugated antibody has been tested by ELISA and is assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room tempe
ELISA Results of Purified Rabbit Anti-Bovine IgG Antibody Peroxidase Conjugated tested against purified Bovine IgG HRP. Each well was coated in duplicate with 1.0 µg of Bovine IgG (p/n 001-0102). The working dilution is 1:102,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), TMB substrate (p/n TMBE-1000).
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