Rabbit IgG anti-Bovine IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Bovine IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-601-4302
• Supplier Catalog Number: 601-4302
• Alternative Catalog Number: ROC-601-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC
• Species Reactivity: Bovine
• Immunogen: Bovine IgG whole molecule
• Conjugation: HRPO
• Alternative Names: rabbit anti-Bovine IgG peroxidase Conjugated Antibody, rabbit anti-Bovine IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Bovine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500
• Application Notes: Anti-Bovine IgG Peroxidase Conjugated antibody has been tested by ELISA and is assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room tempe

Images

ELISA Results of Purified Rabbit Anti-Bovine IgG Antibody Peroxidase Conjugated tested against purified Bovine IgG HRP. Each well was coated in duplicate with 1.0 µg of Bovine IgG (p/n 001-0102). The working dilution is 1:102,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), TMB substrate (p/n TMBE-1000).