Goat IgG anti-Cat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Goat IgG anti-Cat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Artikelnummer: ROC-602-1502
• Hersteller Artikelnummer: 602-1502
• Alternativnummer: ROC-602-1502
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Feline
• Immunogen: Cat IgG whole molecule
• Konjugation: Alk. Phos.
• Alternative Synonym: Goat Anti-Cat IgG Antibody Alkaline Phosphatase Conjugation, Goat Anti-Cat IgG Alk Phos Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Cat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:6,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Cat IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot

Bilder

ELISA of Purified Goat Anti-Cat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Cat IgG. Each well was coated in duplicate with 10 µg of Cat IgG (p/n 002-0102). The working dilution of Cat IgG Alk Phos is 1:2000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and NPP Substrate (p/n NPP-100).