Goat IgG anti-Cat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat IgG anti-Cat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-602-1502
• Supplier Catalog Number: 602-1502
• Alternative Catalog Number: ROC-602-1502
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Feline
• Immunogen: Cat IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: Goat Anti-Cat IgG Antibody Alkaline Phosphatase Conjugation, Goat Anti-Cat IgG Alk Phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Cat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:6,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Cat IgG Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot

Images

ELISA of Purified Goat Anti-Cat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Cat IgG. Each well was coated in duplicate with 10 µg of Cat IgG (p/n 002-0102). The working dilution of Cat IgG Alk Phos is 1:2000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066) and NPP Substrate (p/n NPP-100).