Goat IgG anti-Dog IgG (Fc)-unconj., MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Dog IgG (Fc)-unconj., MinX none
• Artikelnummer: ROC-604-1103
• Hersteller Artikelnummer: 604-1103
• Alternativnummer: ROC-604-1103
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Canine
• Immunogen: Dog IgG F(c) fragment
• Konjugation: Unconjugated
• Alternative Synonym: goat anti Dog IgG F(c) Antibody, goat anti Dog IgG Fc Antibody
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Dog
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Dog IgG F(c) antibody has been tested by ELISA and dot blot and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Bilder

ELISA Results of Purified Goat Anti-Dog IgG F(c) Antibody tested against purified Dog IgG F(c). Each well was coated in duplicate with 10 µg of Dog IgG F(c) (p/n 004-0103) [Red Line] and Dog IgG (p/n 004-0102) [Green Line]. The working dilution of Dog IgG F(c) is 1:48,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Donkey Anti-Goat IgG mx8 HRP conjugated and TMB substrate (p/n TMBE-1000).