Goat IgG anti-Dog IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Goat IgG anti-Dog IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-604-1103
• Supplier Catalog Number: 604-1103
• Alternative Catalog Number: ROC-604-1103
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Canine
• Immunogen: Dog IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: goat anti Dog IgG F(c) Antibody, goat anti Dog IgG Fc Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Dog
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Dog IgG F(c) antibody has been tested by ELISA and dot blot and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

ELISA Results of Purified Goat Anti-Dog IgG F(c) Antibody tested against purified Dog IgG F(c). Each well was coated in duplicate with 10 µg of Dog IgG F(c) (p/n 004-0103) [Red Line] and Dog IgG (p/n 004-0102) [Green Line]. The working dilution of Dog IgG F(c) is 1:48,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Donkey Anti-Goat IgG mx8 HRP conjugated and TMB substrate (p/n TMBE-1000).