Goat IgG anti-Dog IgG (H+L)-HRPO, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Dog IgG (H+L)-HRPO, MinX none
• Artikelnummer: ROC-604-1302
• Hersteller Artikelnummer: 604-1302
• Alternativnummer: ROC-604-1302
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Canine
• Immunogen: Dog IgG, whole molecule
• Konjugation: HRPO
• Alternative Synonym: Goat anti-Dog IgG Antibody Peroxidase Conjugation, Goat anti-Dog IgG HRP Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Dog
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:50,000 - 1:150,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Dog IgG Peroxidase conjugated antibody has been tested by ELISA, This product has been assayed against 1.0 µg of Dog IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate fo

Bilder

ELISA Results of Purified Goat Anti-Dog IgG Antibody Peroxidase Conjugated tested against purified Dog IgG. Each well was coated in duplicate with 1.0 µg of Dog IgG (p/n 004-0102). The working dilution is 1:36,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).