Goat IgG anti-Dog IgG (H+L)-HRPO, MinX none

Product Overview

• Article Name: Goat IgG anti-Dog IgG (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-604-1302
• Supplier Catalog Number: 604-1302
• Alternative Catalog Number: ROC-604-1302
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Canine
• Immunogen: Dog IgG, whole molecule
• Conjugation: HRPO
• Alternative Names: Goat anti-Dog IgG Antibody Peroxidase Conjugation, Goat anti-Dog IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Dog
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000 - 1:150,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: Anti-Dog IgG Peroxidase conjugated antibody has been tested by ELISA, This product has been assayed against 1.0 µg of Dog IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate fo

Images

ELISA Results of Purified Goat Anti-Dog IgG Antibody Peroxidase Conjugated tested against purified Dog IgG. Each well was coated in duplicate with 1.0 µg of Dog IgG (p/n 004-0102). The working dilution is 1:36,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).