Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none

Artikelnummer: ROC-604-4304
Artikelname: Rabbit IgG anti-Dog IgG (F(ab)2)-HRPO, MinX none
Artikelnummer: ROC-604-4304
Hersteller Artikelnummer: 604-4304
Alternativnummer: ROC-604-4304
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Canine
Immunogen: Dog IgG F(ab)2 fragment
Konjugation: HRPO
Alternative Synonym: rabbit anti-dog IgG F(ab)2 Antibody Peroxidase conjugation, rabbit anti-Dog IgG Fab2 fragment HRP conjugated Antibody
Format: IgG
Spezifität: IgG (F(ab')2)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Dog
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:20,000
Anwendungsbeschreibung: Anti-Dog IgG F(ab)2 Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot co
ELISA results of purified Rabbit Anti-Dog IgG F(ab)2 Antibody tested against purified Dog IgG F(ab)2. Each well was coated in duplicate with 1.0 µg of Dog IgG F(ab)2 (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.